ENTERIC SEROGROUPING

Escherichia coli - Shiga-toxin producing

Salmonella

Shigella


 

Escherichia coli  Serotyping (STX positive)

Shigatoxin (STX) producing E. coli infections are also a Wyoming Department of Health Reportable Disease.  Laboratories that isolate STX positive E. coli from a patient are required to send the isolate to WPHL.  STX positive E. coli isolates are then serotyped.  Additionally, Pulsed Field Gel Electrophoresis (PFGE) is performed on the isolate to determine if the patient is a part of an outbreak or a sporadic case.

 

TYPE OF TEST

Agglutination.

SPECIMEN REQUIREMENTS

Pure isolates grown on appropriate media should be submitted to WPHL after apparent growth is observed.

SHIPPING REQUIREMENTS

Ship according to regulations for infectious substances.  Complete and enclose WPHL test request form.

DAYS TEST PERFORMED

Daily, Monday – Friday.

RESULTS AVAILABLE

Upon completion of identification.

REPORTING & INTERPRETATION

ADDITIONAL COMMENTS

WPHL maintains sera for the most common STX positive E. coli serotypes (O26, O45, O103, O104, O111, O121, and O157.  All other isolates are sent to CDC Atlanta for further characterization.

 


 

Salmonella Serogrouping and Serotyping

Salmonellosis is a Wyoming Department of Health Reportable Disease.  Laboratories that isolate Salmonella from a patient are required to send the isolate to WPHL.  Salmonella isolates are then serogrouped and serotyped.  Additionally, Pulsed Field Gel Electrophoresis (PFGE) is performed on the isolate to determine if the patient is a part of an outbreak or a sporadic case.

TYPE OF TEST

Agglutination.

SPECIMEN REQUIREMENTS

Pure isolates grown on appropriate media should be submitted to WPHL after apparent growth is observed.

SHIPPING REQUIREMENTS

Ship according to regulations for infectious substances.  Complete and enclose WPHL test request form.

DAYS TEST PERFORMED

Daily, Monday – Friday.

RESULTS AVAILABLE

Upon completion of identification.

REPORTING & INTERPRETATION

Salmonella serogroups are reported to the submitter.  However, Salmonella serotypes are not usually reported to the submitter, but are available through the Wyoming Epidemiological section.

ADDITIONAL COMMENTS

 


 

Shigella Serogrouping

Shigellosis is a Wyoming Department of Health Reportable Disease.  Laboratories that isolate Shigella from a patient are required to send the isolate to WPHL.  Shigella isolates are then serotyped into Groups A, B, C, or D.  Additionally, Pulsed Field Gel Electrophoresis (PFGE) is performed on the isolate to determine if the patient is a part of an outbreak or a sporadic case.

 

TYPE OF TEST

Agglutination.

SPECIMEN REQUIREMENTS

Pure isolates grown on appropriate media should be submitted to WPHL after apparent growth is observed.

SHIPPING REQUIREMENTS

Ship according to regulations for infectious substances.  Complete and enclose WPHL test request form. 

DAYS TEST PERFORMED

Daily, Monday – Friday.

RESULTS AVAILABLE

Upon completion of test.

REPORTING & INTERPRETATION

Shigella groups are generally thought to be associated with the following species:

Group A - Shigella dysenteriae

Group B - Shigella flexneri

Group C - Shigella boydii

Group D - Shigella sonnei

ADDITIONAL COMMENTS

  

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